Polymerase DNA Directed Alpha 1 (POLa1), Recombinant, Mouse, His-Tag

Catalog Number: USB-049311
Article Name: Polymerase DNA Directed Alpha 1 (POLa1), Recombinant, Mouse, His-Tag
Biozol Catalog Number: USB-049311
Supplier Catalog Number: 049311
Alternative Catalog Number: USB-049311-10,USB-049311-50,USB-049311-100,USB-049311-200
Manufacturer: US Biological
Category: Molekularbiologie
Application: WB
Recombinant protein corresponding to Ala1209~Ser1465 from mouse Polymerase DNA Directed Alpha 1, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~33.5kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight: 33.5
UniProt: P33609
Purity: 95%.
Form: Supplied as a lyophilized powder in 20mM Tris, pH 8.0, 150mM sodium chloride, 0.01% SKL, 1mM DTT, 1mM EDTA, 5% trehalose, Proclin300. Reconstitute with sterile 20mM Tris, pH 8.0, 150mM sodium chloride to 0.1-1mg/ml. Do not vortex.