Avidin D, Fluorescein labeled, having an excitation at 495nm and an emission at about 515nm, is made from highly purified Avidin D by a special labeling technique. High fluorochrome to protein ratio and is the reagent we recommend for routine immuno-fluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. F/P (molar): 2.7 Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form:
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 8.5, 0.08% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
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