Avidin DN is isolated by an affinity chromatographic system that results in a form of avidin which, like Avidin DH, exhibits very low nonspecific binding to nucleic acids. Although the reasons for this property are not fully understood, this feature can be exploited when using biotin-avidin detection systems with biotin-labeled DNA or RNA probes for in situ or nitrocellulose hybridization techniques. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium bicarbonate, 0.05% thimerosal, pH 8.2.
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