Source: Neutralized (pI = 6.3), deglycosylated avidin purified from egg white. Mr/Structure: 60kD tetramer of four identical subunits, each containing a single binding site. Applications: Enzyme-Linked-Immunosorbent-Assay (ELISA) Immunoblotting Immunohistochemistry Recommended Dilution: ELISA: 1:4000-1:8000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified
Form:
Supplied as a liquid in 50% glycerol/50% PBS, pH 7.4. No preservative added. Labeled with HRP.
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