CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (FITC), IgG1, Clone: [1A29], Mouse, Monoclonal100
Biozol Catalog Number:
USB-C2409-48G-FITC
Supplier Catalog Number:
C2409-48G-FITC
Alternative Catalog Number:
USB-C2409-48G-FITC-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, IP
Immunogen:
Rat Ax cells (a HEV derived cell line).
CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. CD29 is reported to inhibit beta 1 integrin mediated adhesion. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: frozen Optimal dilution determined by the researcher Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: I1904-78Y: IgG1, Murine Negative Control (For rat tissue) Recommended secondary antibodies: (Rat Adsorbed) I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
