CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (MaxLight 490), IgG1, Clone: [1A29], Mouse, Monoclona
Biozol Catalog Number:
USB-C2409-48G-ML490
Supplier Catalog Number:
C2409-48G-ML490
Alternative Catalog Number:
USB-C2409-48G-ML490-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, IP
Immunogen:
Rat Ax cells (a HEV derived cell line).
MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. CD29 is reported to inhibit beta 1 integrin mediated adhesion. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: frozen Optimal dilution determined by the researcher Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: I1904-78Y: IgG1, Murine Negative Control (For rat tissue) Recommended secondary antibodies: (Rat Adsorbed) I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
