MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. CD55 is a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. Found specifically at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. This antibody has a functional binding affinity to erythrocytes of 8.7x10e7 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Functional assays: Removal of sodium azide is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3H1564 BRIC 216]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)550.
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