Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5,000-1:100,000 using chromogenic substrates Immunocytochemistry: 1:500-1:5,000 Immunohistochemistry: 1:500-1:5,000 Western Blot: 1:5,000-1:100,000 using chromogenic substrates Western Blot: 1:10,000-1:200,000 using ECL substrates Optimal dilution to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purifed by immunoaffinity chromatography
Form:
Supplied as a powder lyophilized from a solution containing PBS, pH 7.6, and 15mg/ml BSA (IgG-free and protease-free). Labeled with Horseradish Peroxidase (HRP). Reconstitute with 0.5ml ddH2O.
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