Applications: Suitable for use in Immunofluorescence, FLISA and Fluorescent Western Blot. Other applications have not been tested. Recommended Dilutions: FLISA: 1:10,000 -1:50,000 Immunofluoresence: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Excitation Wavelength: 495nm Emission Wavelength: 528nm Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form:
Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC, MW= 390 kD). Reconstitute with 1ml deionized water.
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