Applications: Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Form:
Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide
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