Applications: Suitable for immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:200-1:1000 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by affinity chromatography.
Form:
Supplied as a lyophilized powder from 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Labeled with horseradish peroxidase (HRP) (MW:40kD).
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