Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:50,000-1:1,000,000 Western Blot: 1:10,000-1:100,000 Immunohistochemistry (tissue sections)1:500-1:5000 Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.05% Sodium Azide, 0.01% Thimerosal. Labeled with Biotin.
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