Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:200-1:5000 Western Blot: 1:200-1:5000 Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, protease/IgG free, 0.1% kathon CG. Reconstitute with .55ml sterile dH2O. Labeled with horseradish peroxidase (HRP).
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