Applications: Suitable for use in Flow Cytometry, FLISA, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:5000 Immunofluorescence: 1:50-1:5000 Immunohistochemistry: 1:20-1:2000 Optimal dilutions to be determined by the researcher. Conjugate: Fluorescein-5-isothiocyanate (FITC) Amax = 494nm, Emax = 518nm, F/P Ratio = 10.6:1 Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography. 95% by SDS-PAGE.
Form:
Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, protease/IgG free, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
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