Applications: Suitable for use in FLISA (Fluorescent), Western Blot, Dot Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution: FLISA (Fluorescent): 1:10,000-1:50,000 IF Microscopy: 1:1,000-1:5,000 Flow Cytometry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
99% Purified by affinity chromatography. No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
* VAT and and shipping costs not included. Errors and price changes excepted
