Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:50,000-1:200,000 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin
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