Applications: Suitable for use in FLISA, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: FLISA (Fluorescent): 1:10,000-:50,000 IF Microscopy: 1:1,000-1:5,000 Flow Cytometry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of goat IgG F(ab)2 Absorption Wavelength: 495nm Emission Wavelength: 528nm Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Prepared by affinity chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).. Reconstitute with 1ml sterile ddH2O.
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