IgM antibodies are present as pentamers in the serum and are produced in response to antigens IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques. IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells. IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. Applications: IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcgammaRIV expression and immunoglobulin binding. Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Source: Bovine serum Purity: 95% (SDS-PAGE or HPLC) Concentration: ~1mg/ml (by UV absorbance at 280nm, Ex 0.1% of 1.18) Form: Supplied as a liquid in 0.05M Tris-HCl, 0.15M sodium chloride and 0.01M glycine, pH 7.4, 15mM sodium azide Storage and Stability: May be stored at 4C. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% (SDS-PAGE or HPLC)
Form:
Supplied as a liquid in 0.05M Tris-HCl, 0.15M sodium chloride and 0.01M glycine, pH 7.4, 15mM sodium azide
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