Recombinant protein corresponding to aa25-54 of mouse Insulin-B linked by polypeptide linker (GGGGS) to aa90-110 of mouse Insulin A with a N-terminal His-Tag, expressed in E. coli. UniProt/Accession: P01326. Amino Acid Sequence: FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSIC SLYQLENYCN Preditcted Molecular Weight: 10.3kD Accurate Molecular Weight: 10kD (SDS-PAGE reducing conditions) Predicted Isoelectric Point: 7.1 Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
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