| Recombinant protein corresponding to aa25~54 and aa90-110 linked by a polypeptide linker (GGGGS), (Accession P01323), with two N-terminal Tags, His-tag and T7-tag expressed in E. coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSSIC SLYQLENYCN Predicted Molecular Weight: 9.9kD Subcellular Location: Secreted Predicted isoelectric point: 6.7 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and SDS-PAGE. ther Applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1m sodium azide, 0.01% bromo-phenol bl 33% glycerol. Add 3-5ul/well for mini gels, 7ul/well for large gels. Store at -20 Stable for 6 months after receipt. Note: Thermal stability is described by the los rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradati or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions. Storage and Stability: Lyophilized powder may be stored at -70C. Stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
