SETA (CIN85, Ruk, SH3KBPI) (Biotin), Clone: [5H43 84], Mouse, Monoclonal
Catalog Number:
USB-S1012-10-BIOTIN
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| Article Name: | SETA (CIN85, Ruk, SH3KBPI) (Biotin), Clone: [5H43 84], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-S1012-10-BIOTIN |
| Supplier Catalog Number: | S1012-10-Biotin |
| Alternative Catalog Number: | USB-S1012-10-BIOTIN-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IP, WB |
| Immunogen: | 6His and HA tagged fusion protein corresponding to full length rat SETA. |
| SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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