SETA (CIN85, Ruk, SH3KBPI) (MaxLight 490), Clone: [5H43 84], Mouse, Monoclonal
Catalog Number:
USB-S1012-10-ML490
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| Article Name: | SETA (CIN85, Ruk, SH3KBPI) (MaxLight 490), Clone: [5H43 84], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-S1012-10-ML490 |
| Supplier Catalog Number: | S1012-10-ML490 |
| Alternative Catalog Number: | USB-S1012-10-ML490-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IP, WB |
| Immunogen: | 6His and HA tagged fusion protein corresponding to full length rat SETA. |
| MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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