SFRP2 (Secreted Frizzled-related Protein 2, FRP-2, SARP1, SDF-5, Secreted Apoptosis Related Protein 1), Goat
Biozol Catalog Number:
USB-S1012-87S
Supplier Catalog Number:
S1012-87S
Alternative Catalog Number:
USB-S1012-87S-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
A synthetic peptide from the C-terminus of aa-KRWQKGQREFKR.
Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000. Western Blot: 1-3ug/ml. Approx 38kD band observed in Human Duodenum lysates (calculated MW of 33.5kD according to NP_003004.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See S1012-87S-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
