Sialoprotein, Bone, CT (BSP, BSP-1, OPN, Bone Sialoprotein I, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1) ), IgG1, Clone: [2Q556 ID1.2], Mouse, Monoclonal
Biozol Catalog Number:
USB-S1013-34B
Supplier Catalog Number:
S1013-34B
Alternative Catalog Number:
USB-S1013-34B-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Purified Human Bone Sialoprotein.
Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry: Paraffin embedded sections Optimal dilutions to be determined by the researcher. Positive Control: Human osteoblasts or osteocytes Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[2Q556 ID1.2]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a lyophilized powder from 50mM Tris, pH 7.4, 0.04% Thirmerosal, 0.09% sodium azide. Reconstitute with 100ul sterile ddH2O.
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