Recombinant protein corresponding to Ser20-Gln1641 of human Siglec-1 expressed in NS0 cells
Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using SH-SY5Y human neuroblastoma cell line and human lymph node Neutralization: 1.5-7.5ug/ml (ND50) in the presence of 5ug/ml recombinant human Siglec-1/CD169 Fc Chimera. Optimal dilutions to be determined by the researcher. Storage and Stablility: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
