Siglec-1 cell surface antigen, also known as CD169, is expressed by subsets of cells of the macrophage/ monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Porcine spleen Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
