Siglec-1 cell surface antigen, is a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of Siglec-1 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the Siglec-1 antigen. In healthy tissue no expression occurs. Siglec-1 is a receptor for glycoconjugates containing sialic acid. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:500, 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:50-1:250 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[10B858 ED3]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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