Siglec-1 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Hybridoma: Y3 rat myeloma cells with spleen cells from AO rat. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
