Recombinant protein corresponding to full length human Spt16.
Spt16 is a 120-140kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. Applications: Suitable for use in Immunoprecipitation, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
