Linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A, KLH-conjugated. Species Sequence Homology: primate.
There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: paraffin Western Blot: 0.5ug/ml. Detected SP-A in 10ug of human lung tissue lysate, band at ~35kD. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb I1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb I1904-40E: IgG, H&L (Biotin) Pab Gt x Rb I1904-46P: IgG, H&L (Biotin) Pab Gt x Rb I1904-42T: IgG, H&L (Biotin) Pab Gt x Rb I1904-42V: IgG, H&L (Biotin) Pab Gt x Rb Recommended Control: Human lung tissue lysate Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.