Synthetic peptide corresponding to 14aa near the C-terminus of human Survivin. Species sequence homology: porcine: (92%), bovine: (79%), mouse: (71%) and rat: (71%)
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-5ug/ml Immunocytochemistry: 10ug/ml. Immunofluorescence: 10ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: MOLT4 cell lysate Blocking Peptide: S8500P Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
