Recombinant protein encompassing an N-terminal region of the human Survivin protein.
Survivin, a member of the inhibitors of apoptosis protein (IAP) family, acts as both an anti-apoptosis and a pro-survival factor. Survivin also mediates embryonic submandibular gland epithelial cell survival. It is prominently expressed in embryonic tissues, overexpressed in nearly all common human cancers, and relatively undetectable in normal human tissue. Survivin is involved in cell proliferation, and also interacts with cyclin-dependent kinase 4 (cdk4). Survivin has been implicated in the regulation of cell cycle regulation, and is also highly expressed during G2/M phases of the cell cycle, downregulated in G1 phase, and localized to the mitotic spindle. Survivin overexpression correlates with tumor progression, and has been shown to be a poor prognostic factor in a variety of solid tumors, including colorectal cancer, hepatocellular carcinoma, lung adenocarcinoma, and esophageal cancer. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunoprecipitation (Native): 5-10ug/ IP reaction ELISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: HEK 293 and HeLa cell lysates. Immunoprecipitation: HEK 293 cell extracts Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[6A189]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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