Recombinant protein corresponding to Met55-Thr300 of human Syntaxin 5 isoform 2 expressed in E. coli.
STX5 (Syntaxin 5) is a predicted 41-42kD member of the syntaxin family of proteins. It is a t-SNARE that is widely expressed (but not in striated muscle), and embedded in either the cis-Golgi network (a 35kD form) or the ER (the 42kD form). STX5 is involved in the regeneration and maintenance of the Golgi apparatus after mitosis. Human STX5 is a type IV single-pass transmembrane protein (very long cytoplasmic N-terminus) that is 355aa in length. It contains an ER retrieval signal (Arg4Lys5Arg6), a t-SNARE domain (aa263-325) with a coiled-coil region (aa 287-318), and a C-terminal transmembrane sequence. The 35kD short form exhibits an alternate start site at Met55. Two other potential splice variants show either the Met55, or a Met97 start site coupled to a 19aa substitution for aa 303-355. Over aa55-300, human STX5 shares 94% aa identity with mouse STX5. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
