TACC3 (Transforming Acidic Coiled-coil Containing Protein 3, ERIC-1), Goat
Biozol Catalog Number:
USB-T0300-01
Supplier Catalog Number:
T0300-01
Alternative Catalog Number:
USB-T0300-01-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
WB
Immunogen:
Recombinant protein corresponding to Glu689-Ile838 of human TACC3, expressed in E. coli. Species Sequence Homology: mouse, 79%
Transforming acidic coiled-coil containing protein 3 (TACC3, also know as ERIC-1) is a 92-140kD member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids in length and contains one poly-Serine region (aa155-160) and a TACC coiled-coil domain (aa637-837). There is one potential splice variant that shows a Lys substitution for aa102-462. Applications: Suitable for use in Simple Western and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using Jurkat human acute T cel leukemia cell line and K562 human chronic myelogenous leukemia cell line. Simple Western: 10ug/ml using Jurkat human acute T cell leukemia line and K562 human chronic myelogenous leukemia cell line. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-28C: IgG, H&L (HRP) Pab Dk x Gt I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab Dk x Gt I1904-28K: IgG, H&L (HRP) Pab Rb x Gt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Gt Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
