Talin (TLN, Talin 1, Talin-1, TLN1, ILWEQ, KIAA1027) (MaxLight 550), IgG1, Clone: [97H6], Mouse, Monoclonal
Biozol Catalog Number:
USB-T0750-02A-ML550
Supplier Catalog Number:
T0750-02A-ML550
Alternative Catalog Number:
USB-T0750-02A-ML550-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
IF, IHC, IP, WB
Immunogen:
Human Talin-1
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. Talin-1 is a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:125-1:200 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Immunofluorescence: Requires fixation and permeabilisation using methanol. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
