Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619aa in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642. Over aa25-614, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 20ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with T0850-03A, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[10.32]
Isotype:
IgG2b
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.02% sodium azide.
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