TARC, Human (Thymus and Activation Regulated Chemokine), Rabbit
Biozol Catalog Number:
USB-T1020-05
Supplier Catalog Number:
T1020-05
Alternative Catalog Number:
USB-T1020-05-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Immunogen:
Recombinant protein corresponding to human TARC expressed in E. coli.
Human TARC is a recently discovered protein belonging to the beta-chemokine (C-C) family of cytokines, secreted by activated monocytes, thymic and lymph node dendritic cells. It is chemotactic towards activated T cells (Th2 subset) which express express CCR4 but ineffective on monocytes. Human TARC is an 8.0kD protein containing 71 amino acid residues. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Neutralization: 2-3ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human TARC (100ng/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotylated anti-human TARC (T1020-05A) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human IL-22. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TARC is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted
