Synthetic peptide corresponding to 19aa within the cytoplasmic C-terminus of rat TR2 conjugated to KLH.
Higher vertebrates are believed to possess at least five basic tastes: Sweet, bitter, sour, salty, and unami (the taste of monosodium glutamate). Taste receptor cells that may selectively reside in various parts of the tongue and respond to different tastants and perceive these taste modalities. Circumvallate papillae, found at the very back of the tongue, are particularly sensitive to biter substances. Foliate papillae, found at the posterior lateral edge of the tongue, are sensitive to sour and bitter. Fungiform papillae at the front of the tongue specialize in sweet taste. Recently, two novel taste receptors, TR1 and TR2, have been cloned with distinct topographical distribution in taste receptor cells and taste buds. TRs are members of a new group of 7 TM domain containing GPCR distantly related to other chemosensory receptors (Ca+-sensing receptor (CaSR, a family of putative hormone receptor (V2R), and metabotropic glutamate receptors). TR1 is expressed in all fungiform taste buds, whereas TR2 localized to the circumvallate taste buds. Both receptors do not co-localize with gustducin. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml using control peptide to coat plates at 5-10ug/ml for T1024-03B (affinity purified) or 1ul for T1024-03A (antiserum). Optimal dilutions to be determined by the researcher. Control Peptide: T1024-03 Taste Receptor 2, Rat Control Peptide (TR2) Control Peptide for T1024-03A (antiserum) and T1024-03B (affinity purified) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4.
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