Tau is a heterogeneous group of proteins that range in molecular weight from 50-67kD. They are instumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons but in some neuropathological lesions, it accumulates within the body of the neuron. Tau protein is a microtubule-associated protein predominantly localized to neuronal axons. Tau promotes tubulin polymerization and the stabilization of microtubules. Hyperphosphorylated forms of Tau are the major components of paired helical filaments (PHF) found in the neurofibrillary tangles which characterize Alzheimers disease. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1,500 on 10ug of PC12 lysates Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
