Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: Bovine brain microtubule proteins purified by two cycles of assembly and disassembly (9) are dissolved in SDS-PAGE sample buffer. 5ug of the microtuble preparation per lane is loaded onto a 4% to 20% SDS-PAGE gradient gel along side molecular weight markers (14.3-200kD). After separation by electrophoresis, the proteins are blotted onto nitrocellulose. Tau is detected as a series of 5 bands (52-68kD). Immunohistochemistry: Stains axons in tissue primarily, however in culture Tau expression is not restricted to just axons. Dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimers brain tissue with T1037. This treatment changes the staining pattern of T1037 to include cell bodies, dendrites and axons of neurons. In untreated samples, T1037 stains axons only. Optimal dilutions to be determined by the researcher. Positive Control: IMR5 cells, Human T98G glioblastoma cells or Alzheimers disease brain tissue Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[2Q123 PC1C6]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
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