A 20aa peptide near the carboxy terminal cytoplasmic region of rat Taurine Transporter (1). Species sequence homology: mouse (100%), canine and human (90%), bovine (78%)
Taurine (2-aminoethane sulfonic acid), a beta amino acid with a negatively charged sulfonic group is one of the most abundant amino acids in the brain. It is particularly enriched (millimolar conc.) in excitable tissues, particularly those that generate oxidants. Taurine is produced by cysteine metabolism and obtained from diet as well. It is a chemically inert molecule with a long turnover rate. It is not incorporated into proteins. It is also thought to play a major role in osmoregulation. It is also postulated to play a critical role in neurotransmission, neuromodulation, and membrane stabilization, neuronal differentiation, migration, synaptogenesis, and development. Taurine is transported to various target tissues by Na-dependent high affinity uptake system carried out by two distinct membrane transporter proteins: a high affinity (Km 40uM) taurine transporter (TAU-1 or TAUT-1) and a beta-alanine and taurine transporter protein (Km 4.5uM) known as TAUT-2. Both TAUT-1/2 share significant sequence homology with the GABA and glycine transporters that are characterized by the presence of 12 transmembrane domains. Taurine transporters are found in brain, retina and many other tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Western Blot: 1-10ug/ml (ECL) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
