T-box 1 (Tbx1, T-box family of transcription factors) (Biotin), Rabbit
Biozol Catalog Number:
USB-T1848-24-BIOTIN
Supplier Catalog Number:
T1848-24-Biotin
Alternative Catalog Number:
USB-T1848-24-BIOTIN-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
IHC, IP, WB
Immunogen:
Synthetic peptide corresponding to the internal region of the mouse T-box 1 (Tbx1) protein.
T-box 1 (or Tbx1) is a member of a gene family characterized by the presence of a region known as the T-box, which is a DNA-binding domain homologous to a region found in the protein product of the T gene. The T, or Brachyury, gene was first identified as being responsible for a mutated mouse phenotype with a short, blunt-ended tail. The T-box DNA-binding domain is 173-185 amino acids in length, and demonstrates strong evolutionary conservation across vertebrate species. Seven mouse T-box genes have been identified: Tbx1-Tbx6 and Tbr-1. Tbx1 gene expression has been studied by RTPCR and in situ hybridization (ISH) throughout murine embryonic development, high expression levels correspond with cranial neural crest cell migration (embryonic days 8.5 - 10.5) and therefore suggest an important role in the development of facial and glandular structures of the head and neck region, including the parathyroid and thymus glands. The human ortholog of the mouse Tbx1 gene has been mapped to chromosome region. a region commonly deleted in DiGeorge syndrome (DGS). In humans, DGS is characterized by conotruncal cardiac defects, aplasia or hyperplasia of the thymus and parathyroid glands, palate abnormalities, developmental delay, and craniofacial dysmorphia. Mouse models of heterozygous null Tbx1 mutations produce cardiovascular defects, disrupted neural crest and cranial nerve migration, middle and inner ear defects, and developmental abnormalities, modeling the human DiGeorge syndrome. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
