Recombinant protein corresponding to human TCCR expressed in NSO cells (Q6UWB1).
TCCR, also known as WSX-1 and IL-27 Ra, is a 96kD member of the type I group 2 cytokine receptor family.1-4 The extracellular region of human TCCR shares 63% aa identity with the extracellular domain of mouse IL-27 Ra.2, 3 TCCR is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and native T cells.1, 2, 4, 5 As with many other class I cytokine receptor chains, IL-27 Ra heterodimerizes with gp130, the common receptor subunit of IL-6 family cytokines, to form a functional IL-27 receptor.6 IL-27 only binds to IL-27 Ra/WSX-1/TCCR and not the gp130 subunit.7 Studies using WSX-1 mice reveal that WSX-1 has the ability to suppress T cell hyperactivity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity.4, 8, 9 In particular, WSX-1 is believed to induce IL-12 R on native T cells, predisposing them to a Th1 phenotype. This is offset by its ability to limit IL-12 (and TNF) production by macrophages, and its suppression of IFN-g production. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells from Balb/c mice. Absorption: 488nm Emission: 565-605nm Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
