TCF3 (Transcription Factor E2-alpha, Class B Basic Helix-loop-helix Protein 21, bHLHb21, Immunoglobulin Enhancer-binding Factor E12/E47, Immunoglobulin Transcription Factor 1, Kappa-E2-binding Factor, Transcription Factor 3, TCF-3
Biozol Catalog Number:
USB-T2024-01
Supplier Catalog Number:
T2024-01
Alternative Catalog Number:
USB-T2024-01-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
ELISA, IHC
Immunogen:
E. coli-derived recombinant protein corresponding to Gly100-Glu500 from human TCF-3.
TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml with immersion fixed HeLa human cervical epithelial carcinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
