Synthetic peptide corresponding to C-KAPRARTSPDEDED, from the internal region of human TCF3 (NP_003191.1).
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]) [supplied by OMIM]. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:128,000 Western Blot: 0.01-0.03ug/ml ~75kD band observed in lysates of cell line Daudi (calculated MW of 67.6kD) Immunohistochemistry: 3-5ug/ml In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
