The rat alpha/beta T cell receptor is expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. This clone is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4-CD8-thymocytes are R73 negative. This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (2). Applications: Suitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100, 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:10-1:100 Optimal dilutions to be determined by the researcher. Hybridoma: X63Ag.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[R73]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.4. No preservative added.
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