The T Cell Receptor alpha/beta (TCR) is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, the antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: use 10ul to label 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line. Recommended Negative Controls: Hamster IgG Negative Control Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[H57-597]
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
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