TCR affinity purified from mouse T-cell hybridoma DO-11.10
The alpha/beta TCR is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, this antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry.Other applications have not been tested. Recommended Dilution: Flow Cytometry: 2ug/10e6 cells using final volume of 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[H57-597]
Purity:
Purified
Form:
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
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