White Leghorn chick thymocytes and blood mononuclear cells.
Precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vb1 and Vb2, respectively.4 Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (742%) or CD8 (264%). TCR2+ cells in the spleen also express either CD4 (371%) or CD8 (644%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 716% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker.1-4 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, In ovo suppression of the development of TCR-Vb1 cells, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Immunohistochemistry: Acetone-fixed, frozen section Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[5K82]
Purity:
Purified
Form:
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
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