White Leghorn chick thymocytes and blood mononuclear cells.
This antibody precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vb families, Vb1 and Vb2, respectively.4 Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (742%) or CD8 (264%). TCR2+ cells in the spleen also express either CD4 (371%) or CD8 (644%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 716% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker.1-4 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[TCR2]
Purity:
Purified
Form:
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
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